insulin-like growth factor, IGF-I and IGF-II, are proteins that promote cellular growth and differenctiation of various organs including the kidney. Each peptide interacts with high-afftinity receptors and also has high affinity for family of
insulin-like growth factor binding proteins (IGFBPs). Altered serum and urine IGFBP patterns in children with chronic renal failure (CRF0 have been described. In this study, we have evaluated serum and urine IGFBP profilles in chronic renal
failure
patient (n=10), by using western ligand blot(WLB). Most patients with chronic renal fallure showed decreased intact serum IGFBP3, increased serum IGFBP2, mrkedly increased urinary IGFBP-1 and totally absent urinary IGFBP3.
To evaluate altered IGFBP profiles, we performed IGFBP-3protease assays with sera and urines form renal failure patients and normal controls. Control serum and sera from chronic renal faliure did not showed protease activity (defined by the
ability
to
degrade[125I]-IGFBP-3). The significant protease activity was found in urines from patients. Although control urine had only minor protease activity, the proteolytic pattern of IGFBP-3 was very similar to that found in urine from renal failure
patients.
Although the exact rele of these protease was not established, this protease may play role in the regulation of grwth by modulating IGF availability in a tissue specific manner.
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